Somaclonal variation in banana pdf

A qualitative description and information are given on somaclonal variants of seven in vitro propagated banana cultivars. Somaclonal variation and in vitro mutation induction have been used in sugarcane, banana and potato, with the objective of improving specific characters, mainly the resistance to diseases and other defects that limit the use of some important commercial varieties. The molecular basis of somaclonal variation is not precisely known, but both genetic and epigenetic mechanisms have been proposed. Differences in both the frequency and nature of somaclonal variation may occur when regeneration is achieved from different tissue sources sahijram et al. Biotechnology applications for banana and plantain improvement. Highly differentiated tissues such as roots, leaves, and stems generally produce more variations than explants with preexisting meristems, such as axillary buds and shoot tips duncan 1997. Somaclonal variation in bananas and plantains musa spp. Conversely, high levels of benzyladenine ba cause the number of chromosomes in the banana cultivar williams to. The role of topolins in micropropagation and somaclonal variation of banana cultivars williams and grand naine musa spp. Although, somaclonal variation produces agronomically inferior off types in banana, it can be used to advantage as a source of new variation in musa spp. In 1984, taiwan banana research institute started a selection program for resistance to fusarium wilt in cavendish bananas using somaclonal variation hwang.

Request pdf analyzing somaclonal variation in micropropagated bananas musa spp. Currently, 56 million tissue culture banana plantlets are planted every year. The molecular basis of somaclonal variation is not precisely known, but both genetic and. Somaclonal variation and in vitro mutation induction. Cote fx, perrier x, teisson c 1993a somaclonal variation in musa sp. In banana, somaclonal variants of different type have been reported. Your use of this pdf, the bioone complete website, and all posted and associated content. Pdf induced somaclonal variation in regenerated banana. Somaclonal variation an overview sciencedirect topics. Somaclonal variation created in an experiment conducted with two potatoes solanum tuberosum l. Somaclonal variation in banana and plantain musa species.

Analyzing somaclonal variation in micropropagated bananas. Pdf genomic changes associated with somaclonal variation. Cytogenetic and molecular characterization of the somaclonal variant cien bta03. Somaclonal and phenotypic variation caused by genetic andor epigenetic modifications, are a valuable source of genetic variation to improve desirable polygenetic traits in crops. Plant tissue culture leading to somaclonal variation has been considered as a rapid and reliable approach for improvement of plants as the generated variation can be used either directly or indirectly in a breeding program aimed at crop improvement. Attempts have been made to improve banana by inducing genetic variability by using both physical and chemical mutagens and exploiting the somaclonal variation a few varieties have been released. Analyzing somaclonal variation in micropropagated bananas musa. Somaclonal variation in banana musa acuminate colla cv. Genomic changes associated with somaclonal variation in banana. Conversely, high levels of benzyladenine ba cause the number of chromosomes in the banana cultivar williams to increase 54. In a micropropagation program, where it is of paramount importance to produce truetotype planting material, somaclonal variation of any kind is undesirable. Variation among plants regenerated from tissue culture is termed somaclonal variation.

In any micropropagation program, 35 per cent somaclonal variation is permissible, but in banana, up to 10 per cent is permitted as practiced by commercial micropropagation outfits owing to its flexible genetic makeup of the crop. The role of topolins in micropropagation and somaclonal. Pcr for the identification of dwarf offtypes generated from banana micropropagation. In a micropropagation program, where it is of paramount importance. Qualitative aspects of somaclonal variations in banana propagated. Cavendish banana cultivars resistant to fusarium wilt acquired through.